KMID : 0545120190290050696
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Journal of Microbiology and Biotechnology 2019 Volume.29 No. 5 p.696 ~ p.703
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Characterization and Genomic Analysis of Novel Bacteriophage ¥ÕCS01 Targeting Cronobacter sakazakii
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Ki Gyeong-Hwuii
Kim Jae-Gon Kim Ki-Hwan Lee Jin-Sun Lee Na-Gyeong Lim Tae-Hyun Yoon Sung-Sik
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Abstract
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Cronobacter sakazakii is an opportunistic pathogen causing serious infections in neonates. In this study, a bacteriophage ¥ÕCS01, which infects C. sakazakii, was isolated from swine feces and its morphology, growth parameters, and genomic analysis were investigated. Transmission electron microscopy revealed that ¥ÕCS01 has a spherical head and is 65.74 nm in diameter with a 98.75 nm contracted tail, suggesting that it belongs to the family Myoviridae. The major viral proteins are approximately 71 kDa and 64 kDa in size. The latent period of ¥ÕCS01 was shown to be 60 min, and the burst size was 90.7 pfu (plaque-forming units)/ infected cell. Bacteriophage ¥ÕCS01 was stable at 4?60¡ÆC for 1 h and lost infectivity after 1 h of heating at 70¡ÆC. Infectivity remained unaffected at pH 4?9 for 2 h, while the bacteriophage was inactivated at pH <3 or >10. The double-stranded ¥ÕCS01 DNA genome consists of 48,195 base pairs, with 75 predicted open reading frames. Phylogenetic analysis is closely related to that of the previously reported C. sakazakii phage ESP2949-1. The newly isolated ¥ÕCS01 shows infectivity in the host bacterium C. sakazakii, indicating that it may be a promising alternative to antibacterial agents for the removal of C. sakazakii from powdered infant formulas.
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KEYWORD
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Bacteriophages, Cronobacter sakazakii, Myoviridae, genomic analysis, food safety
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